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experimental_design.pptx

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Experimental Design
?
Experimental design is the manner in which a scientific study is to be carried
out.
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An experiment is aimed at analyzing data and determining outcomes.
?
To conduct a valid scientific experiment whose results are acceptable one
must use a standard experimental design.
?
The experimental design employed determines the validity, reliability and
replicability of the results obtained.
?
The design used helps the scientist conduct the study in a systematic manner
thus ensuring all aspects of the research are considered.
Experiment: Determining the iron content in
froot loops cereal.
?
Iron is one of the key nutrients needed by the body since it forms part of
hemoglobin which deals with gaseous exchange in the body.
?
Foods rich in iron are recommended so as to prevent cases of anemia due to
iron deficiency.
?
In this experiment the iron content in froot loops cereal will be determined.
Equipment required
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200g of froot loops cereal
?
A bowl
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A pestle
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0.1M potassium thiocyanate(KCSN)
?
2M hydrochloric acid
?
Spectrophotometer
Data collection and presentation
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Data will be collected via direct observation.
?
It will be presented in tables, graphs and charts which will enable data
analysis.
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The control test will be measuring the amount of iron in a sample of iron
sulphate which has a known iron mass of 2 grams.
Procedure
?
Crush the froot loops cereal in a bowl using the pestle.
?
Add the crushed cereal into 500ml of 2M HCl.
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Filter the substrate from the mixture.
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Mix the mixture with KSCN.
?
Use spectrophotometer to measure the absorbance of the resulting reddish
brown liquid.
?
Use the absorbance to calculate the iron concentration.
Images
Crushed froot loops
cerial
Images
The KSCN and HCl
mixture forming a
reddish brown
pigment.
Images
Spectrophotometry to
determine absorbance

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